Setting up the Microscope for Thermal Microscopy.
The following information is for upright microscopes only. Inverted microscopes do not require a condenser extension lens.
In order to set up the optimum illumination of your sample when working with transmitted light, (kohler illumination you will need a longer working distance top lens for your condenser.
Just as objective lenses have a working distance, so to do condenser lenses. The objective working distance is defined as the distance from the tip of the objective lens to the point at which the sample is in focus. In the diagram above this distance must be at least 4.5mm.
The condenser working distance is the distance between the surface of the condenser lens and the point at which light from the base of the microscope is focused to a point. In the diagram above this must be at least 12.5mm
In a hotstage the sample is isolated from the objective and condenser by the hotstage chamber and so long working distance lenses are required.
Long working distance objective lenses can be purchased from the microscope manufacturer or we can supply them for you. Linkam makes condenser extension lenses specifically for a variety of condensers from different microscope suppliers.