BCS196 Cryobiology Microscope Cooling Stage

The Linkam BCS196 microscope cooling stage has been at the forefront of cryobiology for over 15 years.

Liquid nitrogen is injected directly into the block by the LNP94/2 cooling pump automatically controlled by the temperature programmer. Temperature feedback is provided by a pt100 platinum resistor to give <0.01s response time.

By precisely controlling the rates of cooling and heating of the microscope stage, ice crystal morphology can be intimately controlled and investigated.

Recycled nitrogen gas is used to purge the microscope stage of moisture and prevent condensation hence enable the capture of clear highly resolved images.

For ice crystal seeding, a cold spot has been provided on one of the cooling pipes allowing nucleated freezing to be initiated.

See sample images taken with BCS196

For high ultra high speed cooling, the microscope stage can be modified so that the sample can be transferred from a copper post onto the pre-cooled silver block. The resulting cooling rate is approximately 5000°C/min. (modified stage seen opposite)

Download Product Data PDF

Low temperature cryobiology (cryo biology) microscope cooling stage
cryo stage modified for vitrification study
  • Temperature Range -196°C to 125 °C
  • Controlled Heating and cooling rates of 0.01 to 130°C/min (can be modified to 5000C/min, contact us for details)
  • Max. 16mm X,Y travel
  • Manual & PC operation(requires linksys software)
  • Designed for use with the Linkam RTVMS software
  • N2 ventilated windows to eliminate condensation
  • Novel low profile lid design for rapid lens change
  • Extremely efficient use of liquid nitrogen
  • Stage body size - 160 x 80 x 24 mm
  • Mounts directly on microscope substage using stage-clamps
  • Objective lens working distance - 0.1mm to 4.5mm
  • Condenser lens minimum working distance 12.7
  • Light aperture - 1.3mm Ø for accurate sample temperatures
  • special lid available for short working distance objectives

    This stage can be used with either the TMS94, CI94 or TP94 temperature controllers.
    In order to cool below ambient you will need the THM-LNP94/2 cooling system
    Also see Optical accessories to see which condenser extension lens you may need.

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