Cutting Edge Cryo-CLEM

Fluorescent beads, 200nm diameter, in ice imaged using the correlative stage (test sample).

Fluorescent beads, 200nm diameter, in ice imaged using the correlative stage (test sample).

At Linkam, we delight in designing temperature control stages at the forefront of new techniques.  We have now in collaboration with some great scientists at LUMC, particularly Erik Bos and Bram Koster, created a stage for cryo-correlative light/electron microscopy (CLEM).

Cryo-CLEM is the correlation of images captured with a cryostage on a fluorescent light microscope and then bringing that same sample to the cryo-Transmission electron microscopy.

To present our design we attended the annual Cryo-Microscopy Group Meeting (CMG) in the City of Birmingham, UK, on 14th November.

One of the speakers at the meeting, Dr Lucy Collinson, spoke positively about some of the preliminary results she has achieved using this stage to study cancer cells. She described that their new correlative stage is being used alongside synchrotrons in Berlin, Barcelona and Oxford to image the cells using a cutting-edge technique called 'soft X-ray tomography'. Soft X-ray tomography allows the scientists to image the structure of the entire cell in 3D.

We look forward to blogging in more detail about this in the future.

Linkam's new phase 2 correlative stage

Linkam's new phase 2 correlative stage

The correlative stage will hold your samples at a stable -196°C. The stage enables you to study your TEM (Transmission Electron Microscope) grid samples at 100x magnification and identify areas of further interest, and also facilitates the movement of these analysed grids to your TEM. With automated liquid nitrogen control, heated optics and a digital display the unit is a compact and efficient system for this work.

The CMG is affiliated to the Royal Microscopical Society and focuses exclusively on promoting low temperature microscopy work. Emergent and established low temperature analytical techniques are becoming increasing relevant in a number of fields including TEM.

For now, we would like to say a big thank for the invitation, the welcome, the lunch and for the boundless enthusiasm of all our customers, both old and new, who we have the pleasure to meet at these exhibitions. Thanks also to Dr Lucy Collinson for her support and feedback.

By Caroline Feltham