Putting Students First

  Linkam's Michael Schwertner demonstrating the CMS196 cryo-correlative stage to participants Tanmay Bharat and Swetha Vijayakrishnan

 

Linkam's Michael Schwertner demonstrating the CMS196 cryo-correlative stage to participants Tanmay Bharat and Swetha Vijayakrishnan

At Linkam we like to encourage and support the next generation of scientists.

Towards the end of September we attended the UK’s first Correlative Light and Electron Microscopy (CLEM) interactive workshop. This was held at the prestigious Francis Crick Institute, in London, and will take place in alternate years with the European EMBO CLEM course in Bristol.

The course was fully booked with eight participants from eight different institutes in the UK working in pairs throughout the week to learn different CLEM workflows. The Linkam CMS196 Cryo-Correlative stage was used during the workshop so students could get practical experience of the system and see how it fits into the Cryo-CLEM workflow.

Cryo-CLEM is a new and emerging technique to combine the individual advantages from both Fluorescence and Electron Microscopy.

Electron microscopy (EM) provides structural information at very high resolution but can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes, whereas fluorescence microscopy is a very sensitive method to detect biological, chemical and genetic processes and events within living cells. This technique is responsible for some of the astonishing photomicroscopy images and videos you can now find.

Cryo-CLEM images the same sample location with both techniques and superimposes the complementing information — it is a powerful tool to gain new biological insights and investigate rare or dynamic molecular events.

Below is a picture of mouse embryonic fibroblast (MEF) cells, prepared by PhD student Patricia Goggin and captured by Linkam's Michael Schwertner during the ‘correlative cryo-fluorescence leading to cryo-electron tomography or cryo soft x-ray tomography’ practical. This cryofluorescence image shows the cell nuclei in blue, mitochondria in green and filamentous actin in red. 

  Cryofluorescence image of mouse embryonic fibroblast cells taken at the workshop. The cell nuclei are shown in blue, mitochondria in green and filamentous actin in red. 

 

Cryofluorescence image of mouse embryonic fibroblast cells taken at the workshop. The cell nuclei are shown in blue, mitochondria in green and filamentous actin in red. 

We had a great time showing off some of the features of our cyro-CLEM stage whilst also continuing to learn about some of its applications, and our thanks go to everyone involved, especially Dr Marie-Charlotte Domart, Dr Raffa Carzaniga, Dr Lucy Collinson and Dr Paul Verkade.

By Frances Coles