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Cryobiology microscope stage system

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BCS196 Cryobiology system

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Features and Benefits

This system has been developed for the specialized field of cryobiology where extremely accurate temperature control is required. The BCS196 cryostage has been at the forefront of cryobiology for over 15 years.

Liquid nitrogen is injected directly into the block by the LNP94/2 cooling pump automatically controlled by the TMS94 temperature programmer. Temperature feedback is provided by a 1/10 din class B pt100 platinum resistor to give <0.01s response time.

By precisely controlling the rates of cooling and heating, ice crystal morphology can be intimately controlled and investigated.

Recycled nitrogen gas is used to purge the stage of moisture and prevent condensation hence enable the capture of clear highly resolved images.

For ice crystal seeding, a cold spot has been provided on one of the cooling pipes allowing nucleated freezing to be initiated.

In order to get highly resolved images, it is important to use a condenser extension lens. See Optical Accessories for more information

For ultra fast cooling, we can modify the BCS196 cryostage with a special push rod and copper post. This enables the Y manipulator to transfer the sample from a copper post to the pre-cooled silver block. This will cool the sample at an approximate rate of 5000C/min and is even faster than submersing the sample in liquid nitrogen.
Visit the Cryobiology Gallery

cryo biology system
Special Option for High Speed Quench Cooling
High speed quench cooling for vitrification study


Specifications

  • Temperature Range -196°C to 125 °C
  • Controlled Heating and cooling rates of 0.01 to 130°C/min
  • Max. 16mm X,Y travel
  • Manual & PC operation(requires linksys software)
  • Designed for use with the Linksys 32 software
  • N2 ventilated windows to eliminate condensation
  • Novel low profile lid design for rapid lens change
  • Extremely efficient use of liquid nitrogen
  • Stage body size - 160 x 80 x 24 mm
  • Mounts directly on microscope substage using stage-clamps
  • Objective lens working distance - 0.1mm to 4.5mm
  • Condenser lens minimum working distance 12.7
  • Light aperture - 1.3mm Ø for accurate sample temperatures
  • special lid available for short working distance objectives

Cryobiology System Includes:

BCS196 cryostage

TMS94 temperature programmer

THM-LNP94/2 liquid nitrogen cooling pump

 

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