Groen et al. explored how a novel 3D cryo-correlative light and X-ray tomography (CLXT) imaging approach could be used to investigate a promising antifibrotic treatment for myocardial fibrosis, using Linkam’s CMS196 to pre-screen vitrified grids.
Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP
Using a Linkam CMS196 stage Beer et al. demonstrated an innovative, targeted cryoCLEM workflow for tissues, in which cryogenic confocal fluorescence imaging of millimetre-scale volumes is correlated to 3D cryogenic electron imaging directed by a patterned surface generated during high-pressure freezing (HPF).
Adaptive 3D cryogenic correlative light imaging of native biology using Linkam’s CMS196 cryo-correlative stage.
Cryogenic light microscopy is a key mapping step in the correlative imaging workflow. Researchers adapted a standard upright widefield microscope into a cryogenic 3D laser-free confocal system using Linkam’s CMS196, to demonstrate the necessary sample preparation steps followed by confocal imaging of biological cells and tissues.
X-ray tomography of cryopreserved human prostate cancer cells: mitochondrial targeting by an organoiridium photosensitiser
Researchers used the Linkam CMS196 for imaging cryopreserved human prostate cancer cells with X-ray tomography.
Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)
Scientists at the The Institute of Cancer Research in London, UK used Linkam’s CMS196 to image mammalian cells with cryo-CLXM microscopy.
Imaging of Vitrified Biological Specimens by Confocal Cryo Fluorescence Microscopy and Cryo FIB /SEM Tomography
Researchers used Linkam’s CMS196 as part of a Confocal Cryo Fluorescence Microscopy setup to image vitrified biological samples.