Groen et al. explored how a novel 3D cryo-correlative light and X-ray tomography (CLXT) imaging approach could be used to investigate a promising antifibrotic treatment for myocardial fibrosis, using Linkam’s CMS196 to pre-screen vitrified grids.
Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP
Using a Linkam CMS196 stage Beer et al. demonstrated an innovative, targeted cryoCLEM workflow for tissues, in which cryogenic confocal fluorescence imaging of millimetre-scale volumes is correlated to 3D cryogenic electron imaging directed by a patterned surface generated during high-pressure freezing (HPF).
Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection
Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
Intrinsic mechanical behavior of femoral cortical bone in young, osteoporotic and bisphosphonate-treated individuals in low- and high energy fracture conditions using a Linkam tensile stage
Investigating a key function for microtubule-associated-protein 6 in activity-dependent stabilisation of actin filaments in dendritic spines using a Linkam Controller.
X‐ray diffraction studies of the structural organisation of prolamellar bodies isolated from Zea mays using a THMS600
Febrile Temperature Elevates the Expression of Phosphatidylserine on Plasmodium falciparum (FCR3CSA) Infected Red Blood Cell Surface Leading to Increased Cytoadhesion
Adaptive 3D cryogenic correlative light imaging of native biology using Linkam’s CMS196 cryo-correlative stage.
Cryogenic light microscopy is a key mapping step in the correlative imaging workflow. Researchers adapted a standard upright widefield microscope into a cryogenic 3D laser-free confocal system using Linkam’s CMS196, to demonstrate the necessary sample preparation steps followed by confocal imaging of biological cells and tissues.
Intrinsic mechanical behaviour of femoral cortical bone in young, osteoporotic and bisphosphonatetreated individuals in low - and high energy fracture conditions
Researchers used Linkam’s Modular Force Stage (MFS) to investigate treatments for osteoporosis in fractures of femoral bones.
X-ray tomography of cryopreserved human prostate cancer cells: mitochondrial targeting by an organoiridium photosensitiser
Researchers used the Linkam CMS196 for imaging cryopreserved human prostate cancer cells with X-ray tomography.
Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)
Scientists at the The Institute of Cancer Research in London, UK used Linkam’s CMS196 to image mammalian cells with cryo-CLXM microscopy.
Imaging of Vitrified Biological Specimens by Confocal Cryo Fluorescence Microscopy and Cryo FIB /SEM Tomography
Researchers used Linkam’s CMS196 as part of a Confocal Cryo Fluorescence Microscopy setup to image vitrified biological samples.
Effect of supercooling and cell volume on intracellular ice formation using Linkam’s FDCS196
Studying the effect of supercooling and cell volume on intracellular ice formation (IIF), using Linkam’s Freeze Drying system the FDCS196.
Using Linkam’s FDCS196 to Investigate How Small Molecule Ice Recrystallisation Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
Researchers at the University of Ottowa, Canada used Linkam’s Freeze Drying system, the FDCS196 to cryopreserve red blood cells with slow cooling rates prior to storage at -80°C.